Deleted
Sample Discontinued
Experience: None
Not Used
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Coding

Part:BBa_K351003:Design

Designed by: Dongchan Yang   Group: iGEM10_KAIST-Korea   (2010-07-22)


Normal Human FGF receptor with human plasma membrane signal peptide


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 586
    Illegal PstI site found at 151
    Illegal PstI site found at 1717
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 586
    Illegal NheI site found at 1368
    Illegal PstI site found at 151
    Illegal PstI site found at 1717
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 586
    Illegal BglII site found at 926
    Illegal BglII site found at 2075
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 586
    Illegal PstI site found at 151
    Illegal PstI site found at 1717
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 586
    Illegal PstI site found at 151
    Illegal PstI site found at 1717
    Illegal NgoMIV site found at 504
    Illegal NgoMIV site found at 1095
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1377
    Illegal BsaI.rc site found at 1865
    Illegal BsaI.rc site found at 2229


Design Notes

Because this sequence contain signal peptide for human plasma membrane, it will not work at other organisms. To express FGFR for other organisms, bind proper signal peptide for membrane of certain organism and FGFR sequence without signal peptide.


Source

Human genome

References